Table 2 Sequences of oligonucleotide primers used to amplify msp-1 block 2 (K1, MAD20 and RO33) allelic types of Plasmodium falciparum [22]
Primers | Sequences | Note |
|---|---|---|
msp1-C1 | 5'-AAGCTTTAGAAGATGCAGTATTGAC-3' | Conserved |
msp1-C2 | 5'-ATTCATTAATTTCTTCATATCCATC-3' | Conserved |
K1a | 5'-GAAATTACTACAAAAGGTGCAAGTG-3' | K1 family-specific |
K1b | 5'-AGATGAAGTATTTGAACGAGGTAAAGTG-3' | K1 family-specific |
MAD20a | 5'-AAATGAAGGAACAAGTGGAACAGCTGTTAC-3' | MAD20 family-specific |
MAD20b | 5'-ATCTGAAGGATTTGTACGTCTTGAATTACC-3' | MAD20 family-specific |
RO33a | 5'-TAAAGGATGGAGCAAATACTCAAGTTGTTG-3' | RO33 family-specific |
RO33b | 5'-CATCTGAAGGATTTGCAGCACCTGGAGATC-3' | RO33 family-specific |