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Figure 1 | Malaria Journal

Figure 1

From: Immunization with different Pf AMA1 alleles in sequence induces clonal imprint humoral responses that are similar to responses induced by the same alleles as a vaccine cocktail in rabbits

Figure 1

Absolute levels of anti-AMA1 antibodies elicited with mixed allele and sequential allele protocols in rabbits. Rabbits in groups 1 - 3 were immunized with three Pf AMA1 allelic antigens (from FVO, HB3 and 3D7 parasite strains) in three different sequences (refer to Table 1). The fourth group of rabbits was immunized with a cocktail of the three Pf AMA1 alleles, while the fifth group was immunized with a mixture of the three Diversity covering proteins (DiCo mix) at all immunization time points. All vaccines were formulated with a modified Freund's adjuvant containing a lipo-polysaccharide from blue-green algae as adjuvant. Antibody titres of sera taken on day 70 were determined by a standardized ELISA with 3D7 (left panel), FVO (middle panel) and HB3 (right panel) AMA1-coated plates. Data is presented on a Log2 scale as dotplots with a boxplot superpose indicating the median, lower and upper quartiles per immunization group. For each capture antigen, plotting symbols represent the antibody titre of individual rabbits within an immunization group.

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