Table 1 Sensitivity and specificity of rt-qPCR Assays using hydrolysis probes and SYBR Green to quantify laboratory prepared dilutions of Plasmodium falciparum

In Water

6.4 × 10⁵ (×3)

24.6 (0.06), 670 000 (28 000)

20.5 (0.10), 740 000 (48 000)

6.4 × 10⁴ (×3)

28.2 (0.09), 60 000 (3 700)

24.6 (0.68), 57 000 (27 000)

6.4 × 10³ (×3)

30.8 (0.50), 9 200 (1 600)

27.6 (0.38), 8 100 (1 600)

6.4 × 10² (×10)

34.6 (0.40), 550 (140)

32.9 (0.57), 550 (200)

6.4 × 10¹ (×10)

37.9 (0.55), 56 (20)

36.1 (1.46), 90 (70)

6.4 (×10)

37.9 (n/a), 52 (n/a)

40.39 (n/a), 4 (n/a)

In Negative Extract

6.4 × 10⁵ (×3)

24.7 (0.02), 650 000 (8 600)

23.0 (0.22), 710000 (100 000)

6.4 × 10⁴ (×3)

28.1(0.20), 62 000 (8 400)

26.6 (0.21), 61 000 (8 200)

6.4 × 10³ (×3)

30.7 (0.41), 6 800 (1 200)

30.1 (0.44), 7,600 (2 600)

6.4 × 10² (×10)

33.5 (0.24), 660 (120)

34.7 (0.37), 410 (120)

6.4 × 10¹ (×10)

37.2 (1.09), 60 (50)

37.2^a (1.22), 90 (90)

6.4 (×10)

37.6 (n/a), 40 (n/a)

39.7^a (1.75), 25 (45)