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Table 1 Sensitivity and specificity of rt-qPCR Assays using hydrolysis probes and SYBR Green to quantify laboratory prepared dilutions of Plasmodium falciparum

From: A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers

Dilutions (replicates) Hydrolysis probe SYBR Green
   Crossing point (SD), Quant value (SD)
In Water 6.4 × 105 (×3) 24.6 (0.06), 670 000 (28 000) 20.5 (0.10), 740 000 (48 000)
  6.4 × 104 (×3) 28.2 (0.09), 60 000 (3 700) 24.6 (0.68), 57 000 (27 000)
  6.4 × 103 (×3) 30.8 (0.50), 9 200 (1 600) 27.6 (0.38), 8 100 (1 600)
  6.4 × 102 (×10) 34.6 (0.40), 550 (140) 32.9 (0.57), 550 (200)
  6.4 × 101 (×10) 37.9 (0.55), 56 (20) 36.1 (1.46), 90 (70)
  6.4 (×10) 37.9 (n/a), 52 (n/a) 40.39 (n/a), 4 (n/a)
In Negative Extract 6.4 × 105 (×3) 24.7 (0.02), 650 000 (8 600) 23.0 (0.22), 710000 (100 000)
  6.4 × 104 (×3) 28.1(0.20), 62 000 (8 400) 26.6 (0.21), 61 000 (8 200)
  6.4 × 103 (×3) 30.7 (0.41), 6 800 (1 200) 30.1 (0.44), 7,600 (2 600)
  6.4 × 102 (×10) 33.5 (0.24), 660 (120) 34.7 (0.37), 410 (120)
  6.4 × 101 (×10) 37.2 (1.09), 60 (50) 37.2a (1.22), 90 (90)
  6.4 (×10) 37.6 (n/a), 40 (n/a) 39.7a (1.75), 25 (45)
  1. a Indicates nonspecific melting peaks of 79°C obtained by melting curve analysis of replicates tested.
  2. n/a = Not Applicable
  3. SD = Standard Deviation