Skip to main content

Advertisement

Table 3 Quantification of parasitaemia in a experimental infected subject, over 10 days and 19 time points with two rt-qPCR assays

From: A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers

  Hydrolysis probe assay SYBR Green assay
Dilutions ( 3 × replicates ) Replicates positive Cp,(SD) Quant value (SD) Replicates positive Cp,(SD) Quant value (SD) Specific melt 82°C
Pre-infection 0/3 ND   3/3 36.8 (0.13)a False Positive 0/3
Day 3 AM 1/3 39.6 (n/a) 18 (n/a) 3/3 37.4 (0.29)a 10 (3) 2/3
Day 3 PM 2/3 38.1 (1.2) 60 (40) 3/3 36.8 (0.44)a 20 (7) 0/3
Day 4 AM 3/3 38.4 (0.59) 40 (20) 3/3 36.8 (0.83)a 20 (15) 1/3
Day 4 PM 0/3 ND   3/3 36.3 (0.23)a False Positive 2/3
Day 5 AM 3/3 33.9 (0.27) 900 (180) 3/3 33.2 (0.45) 320 (100) 3/3
Day 5 PM 3/3 33.2 (0.04) 1400 (40) 3/3 32.3 (0.64) 700 (300) 3/3
Day 6 AM 3/3 37.7 (0.85) 70 (40) 3/3 36.9 (0.80)a 20 (13) 2/3
Day 6 PM 3/3 30.3 (0.14) 10100 (1000) 3/3 29.6 (0.68) 5900 (3000) 3/3
Day 7 AM 3/3 29.2 (0.23) 22000 (3300) 3/3 27.3 (0.30) 32000 (7600) 3/3
Day 7 PM * 3/3 31.2 (0.07) 5600 (270) 3/3 29.8 (0.62) 4900 (2100) 3/3
Day 7 PM+6 hrs 3/3 33.5 (0.55) 1200 (400) 3/3 31.7 (0.19) 1100 (160) 3/3
Day 8 AM 3/3 34.8 (0.13) 460 (40) 3/3 33.0 (0.39) 400 (130) 3/3
Day 8 AM+6 hrs 3/3 35.7 (0.91) 300 (140) 3/3 34.4 (0.30) 130 (30) 3/3
Day 8 PM 3/3 37.1 (1.42) 130 (100) 3/3 34.4 (0.45) 140 (40) 3/3
Day 9 AM 2/3 38.9 (0.38) 30 (20) 3/3 35.0 (0.10)a 80 (6) 2/3
Day 9 PM 1/3 39.35 (n/a) 20 (n/a) 3/3 36.1 (0.44)a 40 (10) 1/3
Day 10 AM 1/3 39.05 (n/a) 30 (n/a) 3/3 37.0 (1.08)a 20 (18) 1/3
Day 10 PM 0/3 ND   3/3 36.8 (0.09)a False Positive 1/3
  1. *Administration of anti-malarial treatment at this time point.
  2. a Indicates nonspecific melting peaks of 79°C obtained by melting curve analysis of replicates tested.
  3. Cp = Crossing point
  4. n/a = not applicable
  5. SD = Standard Deviation
  6. ND = Not Detected