Advertisement
Table 3 Quantification of parasitaemia in a experimental infected subject, over 10 days and 19 time points with two rt-qPCR assays
| Hydrolysis probe assay | SYBR Green assay | ||||||
|---|---|---|---|---|---|---|---|
| Dilutions ( 3 × replicates ) | Replicates positive | Cp,(SD) | Quant value (SD) | Replicates positive | Cp,(SD) | Quant value (SD) | Specific melt 82°C |
| Pre-infection | 0/3 | ND | 3/3 | 36.8 (0.13)a | False Positive | 0/3 | |
| Day 3 AM | 1/3 | 39.6 (n/a) | 18 (n/a) | 3/3 | 37.4 (0.29)a | 10 (3) | 2/3 |
| Day 3 PM | 2/3 | 38.1 (1.2) | 60 (40) | 3/3 | 36.8 (0.44)a | 20 (7) | 0/3 |
| Day 4 AM | 3/3 | 38.4 (0.59) | 40 (20) | 3/3 | 36.8 (0.83)a | 20 (15) | 1/3 |
| Day 4 PM | 0/3 | ND | 3/3 | 36.3 (0.23)a | False Positive | 2/3 | |
| Day 5 AM | 3/3 | 33.9 (0.27) | 900 (180) | 3/3 | 33.2 (0.45) | 320 (100) | 3/3 |
| Day 5 PM | 3/3 | 33.2 (0.04) | 1400 (40) | 3/3 | 32.3 (0.64) | 700 (300) | 3/3 |
| Day 6 AM | 3/3 | 37.7 (0.85) | 70 (40) | 3/3 | 36.9 (0.80)a | 20 (13) | 2/3 |
| Day 6 PM | 3/3 | 30.3 (0.14) | 10100 (1000) | 3/3 | 29.6 (0.68) | 5900 (3000) | 3/3 |
| Day 7 AM | 3/3 | 29.2 (0.23) | 22000 (3300) | 3/3 | 27.3 (0.30) | 32000 (7600) | 3/3 |
| Day 7 PM * | 3/3 | 31.2 (0.07) | 5600 (270) | 3/3 | 29.8 (0.62) | 4900 (2100) | 3/3 |
| Day 7 PM+6 hrs | 3/3 | 33.5 (0.55) | 1200 (400) | 3/3 | 31.7 (0.19) | 1100 (160) | 3/3 |
| Day 8 AM | 3/3 | 34.8 (0.13) | 460 (40) | 3/3 | 33.0 (0.39) | 400 (130) | 3/3 |
| Day 8 AM+6 hrs | 3/3 | 35.7 (0.91) | 300 (140) | 3/3 | 34.4 (0.30) | 130 (30) | 3/3 |
| Day 8 PM | 3/3 | 37.1 (1.42) | 130 (100) | 3/3 | 34.4 (0.45) | 140 (40) | 3/3 |
| Day 9 AM | 2/3 | 38.9 (0.38) | 30 (20) | 3/3 | 35.0 (0.10)a | 80 (6) | 2/3 |
| Day 9 PM | 1/3 | 39.35 (n/a) | 20 (n/a) | 3/3 | 36.1 (0.44)a | 40 (10) | 1/3 |
| Day 10 AM | 1/3 | 39.05 (n/a) | 30 (n/a) | 3/3 | 37.0 (1.08)a | 20 (18) | 1/3 |
| Day 10 PM | 0/3 | ND | 3/3 | 36.8 (0.09)a | False Positive | 1/3 | |
