| |
Hydrolysis probe assay
|
SYBR Green assay
|
|---|
|
Dilutions ( 3 × replicates )
|
Replicates positive
|
Cp,(SD)
|
Quant value (SD)
|
Replicates positive
|
Cp,(SD)
|
Quant value (SD)
|
Specific melt 82°C
|
|---|
|
Pre-infection
|
0/3
|
ND
| |
3/3
|
36.8 (0.13)a
|
False Positive
|
0/3
|
|
Day 3 AM
|
1/3
|
39.6 (n/a)
|
18 (n/a)
|
3/3
|
37.4 (0.29)a
|
10 (3)
|
2/3
|
|
Day 3 PM
|
2/3
|
38.1 (1.2)
|
60 (40)
|
3/3
|
36.8 (0.44)a
|
20 (7)
|
0/3
|
|
Day 4 AM
|
3/3
|
38.4 (0.59)
|
40 (20)
|
3/3
|
36.8 (0.83)a
|
20 (15)
|
1/3
|
|
Day 4 PM
|
0/3
|
ND
| |
3/3
|
36.3 (0.23)a
|
False Positive
|
2/3
|
|
Day 5 AM
|
3/3
|
33.9 (0.27)
|
900 (180)
|
3/3
|
33.2 (0.45)
|
320 (100)
|
3/3
|
|
Day 5 PM
|
3/3
|
33.2 (0.04)
|
1400 (40)
|
3/3
|
32.3 (0.64)
|
700 (300)
|
3/3
|
|
Day 6 AM
|
3/3
|
37.7 (0.85)
|
70 (40)
|
3/3
|
36.9 (0.80)a
|
20 (13)
|
2/3
|
|
Day 6 PM
|
3/3
|
30.3 (0.14)
|
10100 (1000)
|
3/3
|
29.6 (0.68)
|
5900 (3000)
|
3/3
|
|
Day 7 AM
|
3/3
|
29.2 (0.23)
|
22000 (3300)
|
3/3
|
27.3 (0.30)
|
32000 (7600)
|
3/3
|
|
Day 7 PM *
|
3/3
|
31.2 (0.07)
|
5600 (270)
|
3/3
|
29.8 (0.62)
|
4900 (2100)
|
3/3
|
|
Day 7 PM+6 hrs
|
3/3
|
33.5 (0.55)
|
1200 (400)
|
3/3
|
31.7 (0.19)
|
1100 (160)
|
3/3
|
|
Day 8 AM
|
3/3
|
34.8 (0.13)
|
460 (40)
|
3/3
|
33.0 (0.39)
|
400 (130)
|
3/3
|
|
Day 8 AM+6 hrs
|
3/3
|
35.7 (0.91)
|
300 (140)
|
3/3
|
34.4 (0.30)
|
130 (30)
|
3/3
|
|
Day 8 PM
|
3/3
|
37.1 (1.42)
|
130 (100)
|
3/3
|
34.4 (0.45)
|
140 (40)
|
3/3
|
|
Day 9 AM
|
2/3
|
38.9 (0.38)
|
30 (20)
|
3/3
|
35.0 (0.10)a
|
80 (6)
|
2/3
|
|
Day 9 PM
|
1/3
|
39.35 (n/a)
|
20 (n/a)
|
3/3
|
36.1 (0.44)a
|
40 (10)
|
1/3
|
|
Day 10 AM
|
1/3
|
39.05 (n/a)
|
30 (n/a)
|
3/3
|
37.0 (1.08)a
|
20 (18)
|
1/3
|
|
Day 10 PM
|
0/3
|
ND
| |
3/3
|
36.8 (0.09)a
|
False Positive
|
1/3
|
- *Administration of anti-malarial treatment at this time point.
- a Indicates nonspecific melting peaks of 79°C obtained by melting curve analysis of replicates tested.
- Cp = Crossing point
- n/a = not applicable
- SD = Standard Deviation
- ND = Not Detected
