Figure 1

Effect of atorvastatin on endothelial expression of adhesion molecules: HLEC were cultured alone or co-cultured with RBC (hematocrit 5%), or PRBC for 4 h (parasitemia 50%, hematocrit 5%) after 24 hours of pretreatment or not with 1 microM of atorvastatin (A, 1 microM). Then, cells were washed and total RNA was isolated. qPCR was done using primers for amplification of human ICAM-1, VCAM-1, P-selectin and CD36. As internal control, we used HPRT. The expression is shown as fold induction compared to control non-treated cells (n = 6), **P < 0.01; *** P < 0.005.