Detection of STEVORs on the surface of merozoites by flow cytometry. (A) Merozoite preparation stained with Giemsa. (B and C, upper panels) Dot-plots of forward scatter (FSC) to side scatter (SSC), merozoite populations are surrounded by the R1 gate. (B and C, lower panel) Dot-plots of Ethidium bromide- to Alexa 488-derived fluorescence of the gated populations. (C, lower panel) double-positive merozoite population demonstrating surface exposure of STEVORs at the upper right quadrant.