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Table 3 Evaluation of PCR on RDT on 12 RDT brands

From: Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR

   Results for P. falciparum with 21/μl PCR on whole blood Ct 33.52 Results for P. falciparum with 830/μl PCR on whole blood Ct 26.86  
RDT name blood volume Ag detection DNA detection (Ct) Ag detection DNA detection (Ct) RDT fragment used
OptiMAL 10 μl n 34,67 p 30,12 1/3 NC
SDFK50 5 μl p 35,32 p 30,36 1/2 NC + C
SDFK60 5 μl p 35,47 p 30,20 1/2 NC + C
Pan MAL card 5 μl n 35,77 p 30,49 1/2 NC
First response* 5 μl p 35,88 p 31,48 2/3 S/C
ICT 5 μl p 36,74 p 30,18 1/2 NC
ParaHIT 8 μl p 37,56 p 33,20 1/2 C
Binax 15 μl p 37,77 p 30,19 1/2 NC
Core* 5 μl p 38,82 p 32,83 2/3 S/C
Hexagon 5 μl p 39,58 p 34,08 1/2 NC
Paracheck* 5 μl p 40,53** p 37,33 C
Carestart* 5 μl p 0 p 39,21 2/3 S/C
  1. -Antigen detection is considered positive (p) as test line(s) was (were) visible and negative (n) if no test line was visible.
  2. -DNA detection by real-time PCR on RDT is expressed in Cycle threshold (Ct)-values and RDTs were ranked in order of increasing Ct-values.
  3. -RDT fragments used were either one third (1/3) or half (1/2) of the proximal part of the nitrocellulose (NC) strip or (two third of) the sample (S) and/or conjugate (C) pad (see Figure 2).
  4. * RDTs with a plastic seal covering ik the nitrocellulose strip
  5. ** In the first test, no signal was seen (Ct-value 0), only by repetition of the DNA extraction a positive signal (Ct-value 40.53) was observed.