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Table 4 Results of PCR on RDT and presence of antigen test lines for OptiMAL and SDFK60 tested with a panel of clinical samples

From: Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR

    OptiMAL SDFK60
    Correct DNA detection by PCR on RDT Correct antigen detection by RDT Correct DNA detection by PCR on RDT Correct antigen detection by RDT
PCR on whole blood Parasite density Nrs Nrs Nrs Nrs Nrs
P. falciparum only gametocytes 10 10 6 9 9
  1-100 10 10 4 10 10
  101-500 15 15 14 15 15
  501-1000 5 5 5 5 5
  >1000 20 20 20 20 20
P. vivax only gametocytes 1 1 0 1 0
  1 to >1000 10 10 10 10 9
P. ovale 1 to >1000 10 10 2 10 4
P. malariae 1 to >1000 10 10 6 10 6
Total single infections 0 to >1000 91 91 67 90 78
Mixed infections 1 to >1000 5 4 3# 2 1#
After start of treatment 0 15 11 1 7 15
Negative 0 10 10 10 10 10
Total   121 116 81 109 104
  1. PCR on whole blood was the reference method.
  2. # numbers of RDT samples with two positive test lines