Figure 2
From: Plasmodium berghei ANKA infection increases Foxp3, IL-10 and IL-2 in CXCL-10 deficient C57BL/6 mice
qRT-PCR and Western Blot analyses of Foxp3 in CXCL-10-/- and WT mouse brain. CXCL-10-/- and wild type C57BL/6 (n = 5-10) were infected with P. berghei ANKA parasitized blood intra-peritoneally. Control mice were injected with uninfected blood. Infected and uninfected mice were sacrificed at day 2, 4 & 8. Messenger RNA and protein were isolated from brain tissue samples, and qRT-PCR and Western blot were performed using the MyiQ real-time PCR detection system (Bio-Rad, CA USA) with primers for Foxp3 and -actin as internal control and SDS-PAGE Western blot and Foxp3, and α-tubulin antibodies respectively. P. berghei ANKA induced Foxp3 mRNA and protein expressions at day 4 in CXCL-10-/- brain were higher and statistically significant compared with the expression in WT. At day 8 p.i however, P. berghei ANKA induced Foxp3 mRNA and protein were higher in WT brain than in CXCL-10-/. P. berghei ANKA induced early expression of Foxp3 mRNA and protein in CXCL-10-/- than in WT.