Figure 3
From: Plasmodium berghei ANKA infection increases Foxp3, IL-10 and IL-2 in CXCL-10 deficient C57BL/6 mice
(A-C): qRT-PCR analysis of Foxp3 in CXCL-10-/- and WT mouse peripheral blood mononuclear cells (PBMC). CXCL-10-/- and wild type C57BL/6 (n = 10) were infected with P. berghei ANKA parasitized blood intra-peritoneally. Control mice were injected with uninfected blood. Mice were sacrificed at day 2, 4 & 8. Messenger RNA was isolated from brain tissue samples, DNase treated and quantified. One microgram (1 μg) of DNase treated RNA was converted to cDNA and qRT-PCR was performed using the MyiQ real-time PCR detection system (Bio-Rad, CA USA) using primers for FOXP3 and -actin as internal control. P. berghei ANKA induced Foxp3 mRNA expression at day 4 in CXCL-10-/- PBMC was higher and statistically significant compared with WT (Figure 3C).