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Figure 1 | Malaria Journal

Figure 1

From: Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testing

Figure 1

Alterations to the optical bench of a common bench-top flow cytometer which allows detection of depolarized side-scatter. a) The lid of the CyFLow® Blue flow cytometer can be easily removed and filters can be swapped by the operator. b) Light path in conventional filter set-up for detection of Forward Scatter (FSC), Side-Scatter (SSC), green (FL-1), orange (FL-2) and red (FL-3) fluorescence. c) Filter set-up that allows detection of depolarized Side-Scatter instead of FL-2 detection. Squares with broken line indicate dichroic mirrors that need to be changed. Red error shows 50%/50% beam splitter. Other beam splitters which divert more light to the depolarized SSC are possible, such as 90%/10% or even 95%/5%. DM = Dichroic Mirror; BP = Bandpass filter, LP = Longpass filter, numbers indicate wavelength in nm.

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Malaria Journal

ISSN: 1475-2875

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