Figure 3
The response of macrophages from mice infected with P. y 17XL or 17XNL to TLR agonists. Peritoneal macrophages were collected from mice at one, three and five days after being intraperitoneally injected with 2 × 105 nRBCs (N), 2 × 105 P. yoelii 17XL- or 17XNL-infected RBCs. 1 × 106 macrophages were then stimulated with medium (M), LPS (10 μg/ml), Pam3CSK4 (10 μg/ml), or CpG (10 μg/ml) for 24 h, and cell supernatants were collected to detect TNF-α (A) and IL-6 levels (B). Three individual experiments were performed, and pooled data are presented as the mean ± SD (*P < 0.05, **P < 0.01).