Figure 5

Semi-quantitative RT-PCR analysis of TLR intracellular molecules in macrophages from mice infected with P. y 17XL or 17XNL. Peritoneal macrophages were collected from mice at one, three and five days after they were intraperitoneally injected with nRBCs, P. yoelii 17XL- or 17XNL-infected RBCs. Total RNA was then isolated from the peritoneal macrophages, and the intracellular molecules, MyD88, IRAK1, and TRAF-6 were amplified, with G3PDH signal used as an internal control (N = 3; one representative experiment was shown).