Table 1 Oligonucleotide primers used for PCR amplification and DNA sequencing of pfatp6 gene
From: Pfatp6 molecular profile of Plasmodium falciparum isolates in the western Brazilian Amazon
Primer pair | Sequence 5'→3' | Target region (bps) | Base pairs |
|---|---|---|---|
1F (sense) | CATACGATGTTGAGGATGTAC | 28-722 | 694 |
2R (antisense) | GACCTATTTCAGTCTTCATACC | ||
3F (sense) | GAGAATCCTGTTCAGTTGAC | 558-1308 | 750 |
4R (antisense) | ATCCTTCTTCTCCATCATCC | ||
5F (sense) | ACCGTGTTTCATTTGTTTAGAG | 1138-1892 | 770 |
6R (antisense) | CCATTTGTTGTTGCCTGAGC | ||
7F (sense) | AATCACCAAGGGGTATCAAC | 1771-2467 | 711 |
8R (antisense) | ACGTATACCAGCCATATGG | ||
9F (sense) | TTCAAAATATGGGAAAAAGAGCA | 2285-3078 | 805 |
10R (antisense) | TGTTGCTGGTAATCCGTCAG |