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Table 1 Frequency of mutations and/or gene amplification in anti-malarial resistance markers pfmdr1 and pfcrt in Plasmodium falciparum isolates in Yaoundé and Mfou, Cameroon

From: Molecular monitoring of plasmodium falciparum drug susceptibility at the time of the introduction of artemisinin-based combination therapy in Yaoundé, Cameroon: Implications for the future

Genes and alleles Number of samples (%) P
  Both sites Yaoundé Mfou
(Suburb of Yaoundé)
 
pfmdr1 amplification (na = 215, nb = NA)
   1 pfmdr1 copy number 215 (100%) 215 (100%) NA NA NA
   > 1 pfmdr1 copy number 0 (0%) 0 (0%) NA NA  
pfmdr1 codon 86 (na = 201, nb = 209)
   Mutant 86Y haplotype only 328 (80%) 153 (76%) 175 (84%) NS
   Mixed N86 and 86Y haplotypes 53 (13%) 28 (14%) 25 (12%)  
   Wild-type N86 haplotype only 29 (7%) 20 (10%) 9 (4%)  
   Total mutant 86Y haplotypec 381 (93%) 181 (90%) 200 (96%) 0.042 d
pf c rt codon 76 (na = 203, nb = 229)
   Mutant 76T haplotype only 270 (62%) 145 (71%) 125 (55%) < 0.001e
   Mixed K76 and 76T haplotypes 89 (21%) 20 (10%) 69 (30%)  
   Wild-type K76 haplotype only 73 (17%) 38 (19%) 35 (15%)  
   Total mutant 76T haplotypec 359 (83%) 165 (81%) 194 (85%) NS d
pf c rt codon 72 (na = 202, nb = 212)
   Mutant 72S haplotype 0 (0%) 0 (0%) 0 (0%) NS
   Wild-type C72 haplotype 414 (100%) 202 (100%) 212 (100%)  
  1. NA: not analysable because of the limited amount of DNA samples
  2. NS: non significant
  3. a: Number of total samples tested in the centre of Yaoundé
  4. b: Number of total samples tested in the suburb of Yaoundé
  5. c: Total mutant haplotype regroups single mutant haplotype and mixed haplotype, all samples with mixed genotype for the considered allele are classified in mutant group.
  6. d: comparison of total mutant haplotypes versus wild-type haplotypes
  7. e: comparison of mutant, wild-type and mixed haplotypes