Comparison of molecular tests for the diagnosis of malaria in Honduras

Malaria Journal

Table 1 Primers and PCR conditions used for the different molecular tests evaluated

Test (species)	Primer ID	Primer sequence 5′-3′	Annealing temperature	Size (bp)
Test 1 (Pf)*	AL7178	CCGGAAATTCGGGTTTTAGAC	60°C	220
	AL7142	GCTTTGAAGTGCATGTGAATTGTGCAC
Test 2 (Pf)	AL7140	CCATTTTACTCGCAATAACGCTGCAT	57°C	716
	AL7141	CTGAGTCGAATGAACTAGTCGCTAC
Test 3 (Pf/Pv)	AL7178 AL7142	CCGGAAATTCGGGTTTTAGAC GCTTTGAAGTGCATGTGAATTGTGCAC	60°C	333/220
	AL7175	CTGATTTTCCGCGTAACAATG
	AL7074	CAAATGTAGCATAAAAATCYAAG
Test 4 (Pv)*	AL7175	CTGATTTTCCGCGTAACAATG	54°C	333
	AL7074	CAAATGTAGCATAAAAATCYAAG

Table 1 shows the primer ID and sequence and PCR conditions used for the novel molecular test used to detect Plasmodium falciparum (Pf) and/or Plasmodium vivax (Pv). *Primers used in the multiplex Test 3

ISSN: 1475-2875