Figure 1From: A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplatesDetection of parasite metabolic acid production with c-SNARF-1. (A) Fluorescence measurements in microplate wells containing RPMI 1640 with phenol red and 10% serum; 5 μM c-SNARF-1 or erythrocytes (5% haematocrit) were added as indicated. Red and blue bars represent mean ± S.E.M. fluorescence emission at 590 and 645 nm, respectively. Notice the marked increase in fluorescence at each emission wavelength when the dye is present. AU, arbitrary units. (B) The ratio of fluorescence output as a function of pH in complete medium. Identical results were obtained with 1, 5, and 20 μM c-SNARF-1 (not shown). (C-D) Mean ± S.E.M. fluorescence ratios in microplate wells seeded with indicated initial parasitaemia and cultivated for 48 h. Note the greater sensitivity for detection of trophozoite-stage parasites. 1 μM c-SNARF-1, 2% haematocrit.Back to article page