Figure 3

Identification of limiting dilution parasite clones. Fluorescence ratio determinations from a single 96-well microplate, read on days 19 and 21 of limiting dilution culture (top and bottom panels, respectively). Red symbols reflect positive wells that were harvested. Note that the weak positives in the top panel (blue arrows) exhibited greater increases after continued culture (red symbols in the bottom panel); additional weak positives in the bottom panel were also confirmed on subsequent reading (not shown). These positives likely represent medium- to slow-growing clones from the HB3 × Dd2 cross. Negative wells exhibit low variability. Edge wells were not used in this experiment.