Table 1 Operating conditions

1. Capillary rinse

1 M HCl; 2 min; 20 psi

2. Capillary rinse

1 M NaOH; 1 min; 20 psi

3. Capillary rinse

Electrolyte solution; 1 min; 20 psi

4. Sample introduction (anodic side)

Analyte; 3 secondes; 0.3 psi (3.5 nL)

5. Wait

Water; 0 seconde

6. Separation

6 min; 10 kV (500 V.cm-1); 0.17 min ramp voltage; 25°C (i = 50 μA)

7. Detection

UV; 214 nm; spectral bandwith 10 nm; acquisition rate 4 Hz; filter normal

8. Autozero

1 min