Figure 1
From: Intracellular proteolysis of kininogen by malaria parasites promotes release of active kinins
Mass spectrometry spectrum of extracellular medium of Plasmodium falciparum or Plasmodium chabaudi parasites. (A) Isolated Plasmodium chabaudi parasites at the trophozoite stage (104 cells) were incubated with 30 μM of Abz–MISLMKRPPGFSPFRSSRI-NH2 (I) for 10 min, at 37°C and peptides generated are described in the right panel. Kinin peptides detected in (A) are: Lys-BK (II), BK (III) and des-Arg9-BK (IV). (B) Detection of BK (bradykinin) peptide in extracellular medium after incubation (1 h) of Plasmodium chabaudi with 0.35 μM kininogen (HK). (C) BK detection after incubation (1 h) of Plasmodium falciparum (104 cells) with HK (0.35 μM).