Figure 5
From: Intracellular proteolysis of kininogen by malaria parasites promotes release of active kinins
Effect of generated kinins by Plasmodium intracellular hydrolysis on ileum contraction. (A) Guinea pig ileum isotonic contraction measurements induced by kinin generated from HK by Plasmodium chabaudi parasites. Traces of ileal contraction are shown after 5 min. incubations with HK; Plasmodium chabaudi parasites; HK (0.33 μM) and parasites; and HK, parasites, and HOE-140 (20nM). (B) Guinea pig ileum contraction induced by kinin generated by falcipain-2 and 3 hydrolysis from HK. Traces of ileal contraction are shown after 5 min. incubations as labeled, including 0.48 μM HK, 46 nM falcipain-2 (FP-2), 18 nM falcipain-3 (FP-3), and/or 10 μM E-64.