Figure 6

Effect of generated kinins peptides by Plasmodium on HUVEC intracellular Ca²⁺mobilization. Malaria parasites (10⁴ cells) were incubated with 20 μg of kininogen for 30 minutes, and extracellular medium was then added to HUVECs loaded with the Fluo-3 AM fluorescent calcium probe and measured in confocal microscopy. (A) Intracellular calcium fluorescence of cells incubated with HK (20 μg), 10 μl of parasite culture medium without HK (Pf), 10 μl of parasite culture medium after incubation with 1.9 μg/μL of HK for 30 min (Pf + HK) and thapsigargin (THG, 5 μM), a Ca²⁺-ATPase inhibitor. Each line represents results for a single cell. (B) Time lapse calcium fluorescence images of HUVECs, with the times of addition of components indicated.