Figure 8
From: Intracellular proteolysis of kininogen by malaria parasites promotes release of active kinins
Effect of generated kinins on HUVECs intracellular Ca2+in the presence of the B2 receptor antagonist HOE-140. Malaria parasites (104 cells) were incubated with 20 μg of HK for 30 minutes, and extracellular medium was then added in HUVECs loaded with the Fluo-3 AM fluorescent calcium probe and incubated previously for 10 min. with HOE-140 (10 μM). (A) Intracellular calcium fluorescence of cell incubated with HK (20 μg), 10 μl of parasite culture medium without HK incubation (Pf), 10 μl of parasite culture medium after incubation with 1.9 μg/μL of HK for 30 min. (Pf + HK) and thapsigargin (THG 5 μM), a ER Ca2+-ATPase inhibitor. Each line represents results for a single cell; (B) Time lapse calcium fluorescence images of HUVECs, with the times of addition of components indicated.