Amplified region | Primer sequence # | Amplification condition |
---|---|---|
pfcrt gene (codons 32-119) | Primary 20: PFCRT 1F 5'CCGTTAATAATAAATACAGGC-3' PFCRT 1R 5'CTTTAAAAATGGAAGGGTGT 3' Nested 16: PFK76T 5'-5'GGCTCACGTTTAGGTGGA3' PRK76T 5'TGAATTTCCCTTTTTATTTCCAAA 3' | 94°C 5 min; 94°C 30 sec, 56°C 1 min, 60°C 90 sec (40X), 60°C 3 min. 94°C 5 min; 94°C 30 sec, 52°C 45 sec, 72°C 45 sec sec (35X), 72°C 5 min. |
pfcrt gene (codons 181-222) | Primary 20: PFCRT 1F 5'CCGTTAATAATAAATACAGGC-3' PFCRT 1R 5'CTTTAAAAATGGAAGGGTGT 3' Nested 48: PFA220S 5'CTTATACAATTATCTCGGAGCAG3' PRA220S 5'ATAATAAAAACAAAGTTTAAGTGT 3' | Same as for above primary 94°C 5 min; 94°C 30 sec, 51°C 45 sec, 72°C 45 sec sec (35X), 72°C 5 min. |
pfmdr-1 gene (N86Y) | Primary 16: MDR1F 5'ATGGGTAAAGAGCAGAAAGA3' MDR1R 5'AACGCAAGTAATACATAAAGTCA3' Nested 47: MDR1FN 5'AGATGGTAACCTCAGTATCA3' MDR1RN 5'TTACATCCATACAATAACTTG3' | 94°C 5 min; 94°C 30 sec, 54°C 45 sec, 72°C 45 sec sec (35Χ), 72°C 5 min. 94°C 5 min; 94°C 30 sec, 49°C 45 sec, 72°C 45 sec sec (35X), 72°C 5 min. |