Figure 2

Infection dynamics of HB3 and 7C424. In the absence of drug pressure (A-C): A) HB3 (black squares) competitively suppresses 7C424 (blue diamonds). B) No difference is detected betweeHB3 grown in single culture (grey squares) and in mixed culture with 7C424 (closed black squares). C) 7C424 reaches high densities single culture (light blue diamonds) than in mixed culture with HB3 (dark blue diamonds). When drug pressure initiated 72 hours after the start of the assay (D-F): D) 7c424 (blue diamonds) experiences competitive release in a mixed infection with HB3 (black squares) E) No difference is detected between HB3 grown in single culture (open grey squares) and in mixed culture with 7C424 (black squares): F) No difference is detected between 7C424 grown in single culture (open light blue diamonds) and in mixed culture with HB3 (dark blue diamonds). When drug pressure is initiated 120 hours after the start of the assay (G-I): G) 7c424 is competitively suppressed in a mixed infection containing HB3 (black squares) and 7C424 (blue diamonds): H) No difference is detected between HB3 grown in single culture (grey squares) and in mixed culture with 7C424 (black squares). I) 7C424 reaches greater densities when grown in single culture (light blue diamonds) than in mixed culture with HB3 (dark blue diamonds). Initiation of drug pressure is indicated by a black arrow. Parasite densities are shown in genomes per microlitre of culture. Points are means of three replicates, error bars represent standard deviation.