Table 2 Detection of Pfs25 and Pvs25 in artificial mixed DNA templates by single nested RT-PCRs and multiplex nested RT-PCRs
DNA templates (no. of copy) | Single nested RT-PCR | Multiplex nested RT-PCR | ||
|---|---|---|---|---|
| Â | Pfs25 | Pvs25 | Pfs25 | Pvs25 |
Pfs25L (0.1) + Pvs25L (106) | − | + | − | + |
Pfs25L (1) + Pvs25L (106) | − | + | − | + |
Pfs25L (10) + Pvs25L (106) | + | + | + | + |
Pfs25L (102) + Pvs25L (106) | + | + | + | + |
Pfs25L (105) + Pvs25L (106) | + | + | + | + |
Pfs25L (106) + Pvs25L (105) | + | + | + | + |
Pfs25L (106) + Pvs25L (102) | + | + | + | + |
Pfs25L (106) + Pvs25L (10) | + | + | + | + |
Pfs25L (106) + Pvs25L (1) | + | − | + | − |
Pfs25L (106) + Pvs25L (0.1) | + | − | + | − |