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Table 2 Detection of Pfs25 and Pvs25 in artificial mixed DNA templates by single nested RT-PCRs and multiplex nested RT-PCRs

From: Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

DNA templates (no. of copy) Single nested RT-PCR Multiplex nested RT-PCR
  Pfs25 Pvs25 Pfs25 Pvs25
Pfs25L (0.1) + Pvs25L (106) + +
Pfs25L (1) + Pvs25L (106) + +
Pfs25L (10) + Pvs25L (106) + + + +
Pfs25L (102) + Pvs25L (106) + + + +
Pfs25L (105) + Pvs25L (106) + + + +
Pfs25L (106) + Pvs25L (105) + + + +
Pfs25L (106) + Pvs25L (102) + + + +
Pfs25L (106) + Pvs25L (10) + + + +
Pfs25L (106) + Pvs25L (1) + +
Pfs25L (106) + Pvs25L (0.1) + +