Table 4 Diagnostic performance of multiplex nested RT-PCR for Pfs25 and Pvs25 with single nested RT-PCR as reference

Sensitivity

75/77 (97.4)

81/82 (98.9)

Specificity

80/80 (100)

80/81 (98.8)

Likelihood ratio of positive test

*

82.4

Likelihood ratio of negative test

0.03

0.01

Ratio of single nested RT-PCR positives to

9.63

2.73

microscopy positives

Ratio of multiplex nested RT-PCR positives to

9.50

2.73

microscopy positives