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Table 4 Diagnostic performance of multiplex nested RT-PCR for Pfs25 and Pvs25 with single nested RT-PCR as reference

From: Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

  Gametocytes (%)  
  P. falciparum P. vivax
Sensitivity 75/77 (97.4) 81/82 (98.9)
Specificity 80/80 (100) 80/81 (98.8)
Likelihood ratio of positive test * 82.4
Likelihood ratio of negative test 0.03 0.01
Ratio of single nested RT-PCR positives to 9.63 2.73
microscopy positives   
Ratio of multiplex nested RT-PCR positives to 9.50 2.73
microscopy positives   
  1. * Denominator is zero.