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Table 5 Prevalence of P. falciparum and P. vivax gametocytes among isolates collected in dry and wet seasons

From: Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

Diagnostic method Dry season Wet season p value
  n % positive* n % positive*  
Microscopy      
P. falciparum gametocyte 3 6.4 3 7.7 0.81
P. vivax gametocyte 17 34 13 32.5 0.88
Single nested RT-PCR      
P. falciparum gametocyte 40 85.1 35 89.7 0.52
P. vivax gametocyte 46 92 36 90 0.74
Multiplex nested RT-PCR      
P. falciparum gametocyte 42 89.4 35 89.7 0.95
P. vivax gametocyte 46 92 36 90 0.74
18S rRNA PCR      
P. falciparum 47 39
P. vivax 50 40
  1. * total number of positives for a given species by 18S rRNA PCR.
  2. - denotes not applicable.