Table 5 Prevalence of P. falciparum and P. vivax gametocytes among isolates collected in dry and wet seasons

Microscopy

P. falciparum gametocyte

3

6.4

3

7.7

0.81

P. vivax gametocyte

17

34

13

32.5

0.88

Single nested RT-PCR

P. falciparum gametocyte

40

85.1

35

89.7

0.52

P. vivax gametocyte

46

92

36

90

0.74

Multiplex nested RT-PCR

P. falciparum gametocyte

42

89.4

35

89.7

0.95

P. vivax gametocyte

46

92

36

90

0.74

18S rRNA PCR

P. falciparum

47

−

39

−

−

P. vivax

50

−

40

−

−