PCR detection of malaria parasites in desiccated Anopheles mosquitoes is uninhibited by storage time and temperature

Table 1 The effects of temperature and time on real-time PCR detection of Plasmodium DNA

Study Condition	Partial Sum of Squares	Degrees of Freedom	F Statistic	P -Value
Model	169.45	12	1.77	0.062
Storage Time	42.84	3	1.78	0.154
Storage Temperature	97.49	3	4.06	0.009
Time*Temperature Interaction	18.83	6	0.39	0.883
Residual	912.02	114
Total	1081.48	126

Two-way factorial ANOVA analysis revealed no significant associations between time, or the interaction of time and temperature, on the ability to PCR detect Plasmodium parasites in infected mosquitoes.
Adjusted R-squared=0.068.
Number of Observations=127.

ISSN: 1475-2875