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Figure 1 | Malaria Journal

Figure 1

From: Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay

Figure 1

Inactivation of PfSHMT (A, B) and PvSHMT (C, D) by thiosemicarbazide.

(A and C) show semi-logarithmic plots of residual activities (ln V/V0) versus incubation times at different thiosemicarbazide concentrations. The thiosemicarbazide concentrations used to inactivate PfSHMT (A) were 0.06 mM (), 0.125 mM (▪), 0.25 mM (), 0.5 mM (□), and 1 mM (), while those for PvSHMT (C) were 0.03 mM (), 0.06 mM (▪), 0.125 mM (), 0.25 mM (□), and 0.5 mM (). (B and D) show plots of the observed rate constants (kobs) calculated from the slopes in A and C, respectively, versus thiosemicarbazide concentrations. Based on Equation 1, KI for Pf- and PvSHMT were 0.36 ± 0.07 mM and 0.21 ± 0.08 mM, whereas kinact of Pf- and PvSHMT were 0.0014 ± 0.001 s-1 and 0.0015 ± 0.002 s-1, respectively.

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