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Figure 2 | Malaria Journal

Figure 2

From: Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay

Figure 2

Absorption spectrum changes upon the addition of 10 mM thiosemicarbazide into PvSHMT (A) and into free PLP (B) in Buffer A at 25°C for 50 min.

For (A): (−), (), and () represent spectra of PvSHMT (λmax 422 nm), PvSHMT in the presence of thiosemicarbazide (λmax 392, 451 and 482 nm), and PvSHMT in the presence of thiosemicarbazide and 1% SDS (λmax 312 and 388 nm). For (B): (−), (), and () are spectra of PLP (λmax 328 and 388 nm), PLP in the presence of thiosemicarbazide (λmax 312 and 388 nm), and PLP in the presence of thiosemicarbazide and 1% SDS (λmax 312 and 388 nm).

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