Figure 2From: Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay Absorption spectrum changes upon the addition of 10 mM thiosemicarbazide into PvSHMT (A) and into free PLP (B) in Buffer A at 25°C for 50 min. For (A): (−), (○), and (●) represent spectra of PvSHMT (λmax 422 nm), PvSHMT in the presence of thiosemicarbazide (λmax 392, 451 and 482 nm), and PvSHMT in the presence of thiosemicarbazide and 1% SDS (λmax 312 and 388 nm). For (B): (−), (○), and (●) are spectra of PLP (λmax 328 and 388 nm), PLP in the presence of thiosemicarbazide (λmax 312 and 388 nm), and PLP in the presence of thiosemicarbazide and 1% SDS (λmax 312 and 388 nm).Back to article page