Figure 5

Emission spectra of PLP, Pf- and PvSHMT in the absence and presence of ligands in buffer A at 25°C. (A) Free PLP (−−−−), PfSHMT (●) and PvSHMT (○) at an equivalent concentration (~ 23 μM) were excited at 388, 420 and 422 nm, respectively, and the maximum emission peaks were 494, 506 and 510 nm, respectively. (B and C) Spectra of PfSHMT alone (●), PfSHMT with L-serine (■, D-serine (✞), L-alanine (△), glycine (□), folinic acid (▲), and glycine which was later treated with folinic acid (+). (D and E) Spectra of PvSHMT alone (○), PvSHMT with L-serine (■), D-serine (✞), L-alanine (△), glycine (□), folinic acid (▲), and glycine which was later treated with folinic acid (+).