Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay

Table 1 Comparison of Plasmodium SHMT production from auto-induction and LB-IPTG media

Properties	PfSHMT		PvSHMT
	LB-IPTG^a	Auto-induction	LB-IPTG^b	Auto-induction
Cell paste (g/litre media culture)	5.13	14.30	3.62	17.26
Specific activity of SHMT in crude lysate (unit/mg protein)	0.02	0.08	0.16	0.18
Total amount of purified protein (mg protein/g cell paste)	0.69	0.52	1.44	1.70
Total amount of purified protein (mg/litre media culture)	3.53	7.38	10.48	29.29

a and b; data from [6] and [7], respectively.
The data shown are derived from a single protein preparation. The values reported are reproducible well in routine protein preparations and deviate less than 20%.

ISSN: 1475-2875