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Table 2 Steady-state kinetic parameters of PvSHMT by SHMT-MTHFR and SHMT-MTHFD assays

From: Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay

Coupling system Steady-state kinetic parameters    Reference
  Km (mM)   kcat (s-1)  
  L-serine THF   
#MTHFR 0.18 ± 0.03 0.14 ± 0.02 0.98 ± 0.06 [7]
*MTHFD 0.19 ± 0.02 0.09 ± 0.02 1.26 ± 0.13 Present work
  1. # racemic [6R,S] THF was used.
  2. *[6 S]-THF was used and the reported kcat (apparent value) was from the experiment carried out keeping the concentration of L-serine fixed at 2 mM and varying THF between 0.025-0.4 mM.