Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay

Table 2 Steady-state kinetic parameters of PvSHMT by SHMT-MTHFR and SHMT-MTHFD assays

Coupling system	Steady-state kinetic parameters			Reference
	K_m (mM)		k_cat (s^-1)
	L-serine	THF
^#MTHFR	0.18 ± 0.03	0.14 ± 0.02	0.98 ± 0.06	[7]
*MTHFD	0.19 ± 0.02	0.09 ± 0.02	1.26 ± 0.13	Present work

^# racemic [6R,S] THF was used.
*[6 S]-THF was used and the reported k_cat (apparent value) was from the experiment carried out keeping the concentration of L-serine fixed at 2 mM and varying THF between 0.025-0.4 mM.

ISSN: 1475-2875