Coupling system
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Steady-state kinetic parameters
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Reference
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Km (mM)
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kcat (s-1)
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L-serine
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THF
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#MTHFR
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0.18 ± 0.03
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0.14 ± 0.02
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0.98 ± 0.06
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[7]
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*MTHFD
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0.19 ± 0.02
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0.09 ± 0.02
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1.26 ± 0.13
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Present work
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- # racemic [6R,S] THF was used.
- *[6 S]-THF was used and the reported kcat (apparent value) was from the experiment carried out keeping the concentration of L-serine fixed at 2 mM and varying THF between 0.025-0.4 mM.