Ad5-CSP/Ad5-CSP vaccination resulted in higher percentage of tetramer positive CD8+T cells than Ad4-CSP/Ad5-CSP in the spleen. Splenocytes and PBMCs were collected two weeks after final vaccination. All vaccination regimens resulted in significantly higher percentage of CD3+ CD8+ NYDNAGTNL tetramer positive T cells in the spleen (A) and circulating blood (B) as measured by flow cytometry, cells were stained with CD8-Alexa flour700, CD3-APC-Cy7, and CSP (NYD)-Tetramer-PE. Ad5-CSP/Ad5-CSP stimulated a higher percentage of CD3+ CD8+ tet+ than Ad4-CSP/Ad5-CSP treated animals in the spleen (A) and higher percentage of CD3+ CD8+ tet+ than Ad4-CSP/Ad4-CSP in the circulating blood (B). Bars represent ± standard error. Statistical analysis was completed using One Way ANOVA with Student-Newman-Keuls post-hoc test, *, **, *** denotes significance over naïve, P < 0.05, P < 0.01, P < 0.001.