All vaccinations stimulated significantly higher anti-CSP total IgG than unvaccinated and AD4-CSP/Ad4-CSP vaccination in Ad naïve animals. Plasma was collected 14 days post the final vaccination. Plasma was diluted 1:100, 1:200, and 1:400 and measured for total IgG against CSP by ELISA. Bars represent ± standard error. Statistical analysis was completed using One Way ANOVA with Student-Newman-Keuls post-hoc test, *, **, *** denotes significance over naïve, P < 0.05, P < 0.01, P < 0.001. ### denotes significance over Ad4-CSP/Ad4CSP treatment, P < 0.001.