All vaccinations in Ad5 immune animals resulted in significantly higher percentages of CD8+CSP tetramer positive cells than unvaccinated Ad5 immune animals. Splenocytes and PBMCs were collected two weeks after final vaccination. All vaccination regimens resulted in significantly higher percentage of CD3+ CD8+ NYDNAGTNL tetramer positive T cells in the spleen (A) and circulating blood (B) as measured by flow cytometry, cells were stained with CD8-Alexa flour700, CD3-APC-Cy7, and CSP (NYD)-Tetramer-PE. Bars represent ± standard error. Statistical analysis was completed using One Way ANOVA with Student-Newman-Keuls post-hoc test, * denotes significance over naïve, P < 0.05.