Figure 4

CM induces pathological changes in myelin sheaths in the CC. (A, B) The CC myelin sheaths in UninfSal (A) and UninfEPO (B) had normal appearance and compacted closely around the axon. In InfSal the myelin sheaths in the CC had several abnormalities (C, D). (E) In InfEPO the degree of abnormality was reduced. The most common abnormality was myelin splitting, which was also occasionally seen in uninfected mice (arrow in A, B, C, D and E). In infected mice, blebs of the myelin sheath (asterisks in C), complete, focal loss (arrowhead in C and D), and low electron density (L in D) were frequently seen. Increased periodicity was seen in InfSal and InfEPO mice (P in E). The denotations do not comprise all abnormalities present in each micrograph. Scale bars (A-E) correspond to 1 μm. (F) Quantification of the total number of abnormalities in the CC revealed a significantly higher number in InfSal mice as shown in the strip chart (*, p < 0.001). Each dot denotes the total number in one animal. The horizontal bars mark the mean values and the whiskers the SD.