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Figure 2 | Malaria Journal

Figure 2

From: Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae s. l.

Figure 2

AS-LAMP detection of the kdr-w mutation in plasmid DNA. (A) Amplification of the target sequence (plasmid) with the wild-type primer set monitored by a real-time turbidimeter (turbidity at 650 nm); wild-type plasmid (green), kdr-w plasmid (red), and negative control (grey). (B) Agarose-gel electrophoresis of the AS-LAMP-amplified products from (A). Lane 1, DNA markers; 2, negative control; 3, wild-type plasmid; 4, kdr-w plasmid. (C) Amplification of the target sequence (plasmid) with the kdr-w primer set monitored by a real-time turbidimeter (turbidity at 650 nm); wild-type plasmid (green), kdr-w plasmid (red), and negative control (grey). (D) Agarose-gel electrophoresis of the AS-LAMP-amplified products from (C). Lane 1, DNA markers; 2, negative control; 3, kdr-w plasmid; 4, wild-type plasmid.

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