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Figure 3 | Malaria Journal

Figure 3

From: Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae s. l.

Figure 3

AS-LAMP detection of the kdr-w mutation in mosquito genomic DNA. (A) Amplification of the target sequence (mosquito genomic DNA) with the wild-type primer set monitored by a real-time turbidimeter (turbidity at 650 nm); +/+ (green), kdr-w/+ (violet), kdr-w/kdr-w (red), and negative control (grey). (B) Agarose-gel electrophoresis of the AS-LAMP-amplified products from (A). Lane 1, DNA markers; 2, negative control; 3, +/+; 4, kdr-w/+; 5, kdr-w/kdr-w. (C) Amplification of the target sequence (mosquito genomic DNA) with the kdr-w primer set monitored by a real-time turbidimeter (turbidity at 650 nm); +/+ (green), kdr-w/+ (violet), kdr-w/kdr-w (red), and negative control (grey). (D) Agarose-gel electrophoresis of the AS-LAMP-amplified products from (C). Lane 1, DNA markers; 2, negative control; 3, kdr-w/kdr-w; 4, kdr-w/+; 5, +/+.

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