Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae s. l.

Table 1 Sensitivity and specificity of the AS-PCR and AS-LAMP methods for kdr mutation detection

Genotype^*	Detection Method		AS-LAMP	Sensitivity (95% CL)	AS-LAMP	Specificity (95% CL)	AS-LAMP
Genotype^*	Sequencing	AS-PCR	AS-LAMP	AS-PCR	AS-LAMP	AS-PCR	AS-LAMP
kdr-w/kdr-w	20	20	23	0.83 (0.64 - 0.93)	0.92 (0.74 - 0.98)	1 (0.96 - 1)	0.99 (0.94 - 1)
kdr-w/+	26	18	20	0.94 (0.73 - 0.99)	0.94 (0.73 - 0.99)	0.98 (0.92 - 0.98)	0.96 (0.90 - 0.98)
+/+	70	73	70	0.93 (0.85 - 0.97)	0.99 (0.92 - 1)	0.94 (0.83 - 0.98)	1 (0.92 - 1)
Unidentified	4	9	7	-	-	-	-
Total	120	120	120	-

Genotypes were analysed by sequencing, AS-LAMP, and AS-PCR. The sensitivity and specificity of the AS-PCR and AS-LAMP methods relative to sequencing are expressed as proportions, and their 9~~confidence limits (CL) are shown in parenthesis.

ISSN: 1475-2875