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Table 1 Oligonucleotide primers used for amplification of different fragments of var genes

From: Genetic diversity of expressed Plasmodium falciparum var genes from Tanzanian children with severe malaria

var Gene region Size of amplified product Tanneal Name of primer Primer sequence Source
DBL1α ~500 54 DBLα-5′ 5′-GCACGAAGTTTTGCAGATAT(A/T)GG-3′3′- [23]
    DBLα-3′ AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′  
DBL1α-CIDR 1.5 kb 52 DBLα-5′ 5′-GCACGAAGTTTTGCAGATAT(A/T)GG-3′ [23]
    CIDR1.1-3′ 3′-T(C/G/T)TAGTAATTTATC(A/C/T)ATTGT-5′  
    CIDR1.2-3′ 3′-T(C/G/T)TAATAAGAATTCGATTGC-5′  
upsA 5′UTR- DBL1α 1.2 kb 54 upsA-5′ 5′-ATTA(C/T)ATTTGTTGTAGGTGA-3′  
    DBLα-3′ 3′-AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′  
upsB 5′UTR- DBL1α 1.3 kb 52 17DBLα-5′ 5′-ATGTAATTGTTGTTTTTTTTTTTGTTAGAATATTTAAA-3′  
    DBLα-3′ 3′-AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′  
psC 5′UTR- DBL1α 1.3 kb 54 5B1-5′ 5′-CACATATARTACGACTAAGAAACA-3′ [15]
    DBLα-3′ 3′-AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′ [23]