Table 1 Oligonucleotide primers used for amplification of different fragments of var genes

DBL1α

~500

54

DBLα-5′

5′-GCACGAAGTTTTGCAGATAT(A/T)GG-3′3′-

[23]

DBLα-3′

AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′

DBL1α-CIDR

1.5 kb

52

DBLα-5′

5′-GCACGAAGTTTTGCAGATAT(A/T)GG-3′

[23]

CIDR1.1-3′

3′-T(C/G/T)TAGTAATTTATC(A/C/T)ATTGT-5′

CIDR1.2-3′

3′-T(C/G/T)TAATAAGAATTCGATTGC-5′

upsA 5′UTR- DBL1α

1.2 kb

54

upsA-5′

5′-ATTA(C/T)ATTTGTTGTAGGTGA-3′

DBLα-3′

3′-AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′

upsB 5′UTR- DBL1α

1.3 kb

52

17DBLα-5′

5′-ATGTAATTGTTGTTTTTTTTTTTGTTAGAATATTTAAA-3′

DBLα-3′

3′-AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′

psC 5′UTR- DBL1α

1.3 kb

54

5B1-5′

5′-CACATATARTACGACTAAGAAACA-3′

[15]

DBLα-3′

3′-AA(A/G)TCTTC(T/G)GCCCATTCCTCGAACCA-5′

[23]