Placental histology of labyrinth zone and morphometric analysis. Representative photomicrograph of H&E stained placental sections of non-infected (NI) (A), K173 (B), NK65 (C) and ANKAΔpm4 infected pregnant mice (D). (E) Histological sections show necrotic foci (arrow) and thrombi (arrowhead) in placentas infected with ANKAΔpm4 (upper picture) and K173 (lower picture) parasites. (F) Relative quantification of vascular space using an automated morphometric procedure in H&E stained sections evaluated in placental sections. Mice were infected i.v. with 106P. berghei K173 or NK65 IE (G13) or ANKAΔpm4 IE (G10); placentas were excised on G18 (K173 and NK65) or G19 (ANKAΔpm4). Scale bar: 20 μm; in (F) line refers to mean; *p<0.05;**p<0.01; ***p<0.001.