Placental gene expression of inflammatory factors. Placentas were collected at G18 (K173 and NK65) or G19 (ANKAΔpm4) and RNA expression of Ccl2, Ccl3, Tlr2, Tlr4 and Tnfα were evaluated by qReal Time PCR. Relative quantification (RQ) was obtained with normalization by GAPDH. Results are plotted as fold change against non-infected controls collected at the same gestational day (G18 versus K173 or NK65 and G19 versus ANKAΔpm4); Line refers to median values. Kruskal-Wallis Test **p<0.01; ***p<0.001 refers to differences between different parasite lines; Unpaired t test #p<0.05; ##p<0.01; ###p<0.001 compares each parasite line to its respective non-infected control.