Figure 1From: Flow cytometric readout based on Mitotracker Red CMXRos staining of live asexual blood stage malarial parasites reliably assesses antibody dependent cellular inhibitionFlow cytometric analysis of Plasmodium falciparum asexual stages stained with CMXRos. Fluorescence intensity distribution of uninfected erythrocytes (A), and asynchronous NF54 P. falciparum-infected erythrocytes (B). The ordinate shows red fluorescence emission from CMXRos (FL3), and the abscissa displays the forward light scatter signal intensity. Representative microscopic images of Giemsa-stained (panels ‘a’ and ‘d’), DAPI-stained (panels ‘b’ and ‘e’) and Mitotracker Red CMXRos-stained (panels ‘c’ and ‘f’) parasites are shown below the graphs. Time course of intra-erthrocytic P. falciparum development (C). Samples were from a highly synchronized culture of P. falciparum NF54 at ~10 hours, ~28 hours, and ~38 hours post invasion and parasitaemia was determined by flow cytometry after CMXRos staining (closed circles) and by microscopy of Giemsa-stained smears (open squares). Representative images of Giemsa-stained (panels ‘g’, ‘h’ and ‘i’), DAPI -stained (panels ‘j’, ‘k’and ‘l’) and Mitotracker Red CMXRos-stained (panels ‘m’, ‘n’ and ‘o’) parasites are shown below the graph. Scale bars are equal to 5 μm (magnification100x).Back to article page