Flow cytometric analysis of Plasmodium falciparum asexual stages stained with CMXRos. Fluorescence intensity distribution of uninfected erythrocytes (A), and asynchronous NF54 P. falciparum-infected erythrocytes (B). The ordinate shows red fluorescence emission from CMXRos (FL3), and the abscissa displays the forward light scatter signal intensity. Representative microscopic images of Giemsa-stained (panels ‘a’ and ‘d’), DAPI-stained (panels ‘b’ and ‘e’) and Mitotracker Red CMXRos-stained (panels ‘c’ and ‘f’) parasites are shown below the graphs. Time course of intra-erthrocytic P. falciparum development (C). Samples were from a highly synchronized culture of P. falciparum NF54 at ~10 hours, ~28 hours, and ~38 hours post invasion and parasitaemia was determined by flow cytometry after CMXRos staining (closed circles) and by microscopy of Giemsa-stained smears (open squares). Representative images of Giemsa-stained (panels ‘g’, ‘h’ and ‘i’), DAPI -stained (panels ‘j’, ‘k’and ‘l’) and Mitotracker Red CMXRos-stained (panels ‘m’, ‘n’ and ‘o’) parasites are shown below the graph. Scale bars are equal to 5 μm (magnification100x).