Agreement between flow cytometric and microscopic readouts of parasitaemia by Bland Altman statistical test. Differences in % parasitaemia are plotted over the average % parasitaemia measurements. Parasitaemia was determined upon serial dilutions of highly synchronized asexual blood-stage cultures of P. falciparum NF54 at the ring stage (A) or late trophozoite and schizont stages (B) by CMXRos staining and flow cytometry and by microscopy. Dotted lines represent upper and lower 95% limits of agreement between the two techniques. The data is representative from two independent experiment sets.