Figure 4

Reproducibility of ADCI assays applying flow cytometric readout. SGI values obtained with malaria immune IgG (1 mg/ml) and malaria naïve IgG (1 mg/ml) in 12 independent assays (A). Ten assays were performed with monocytes from one donor and two assays were performed with monocytes from another donor. SGI values at two concentrations (125 μg/ml and 1 mg/ml) of malaria immune IgG in five independent experiments (B). SGI values obtained at three concentrations (12.5 μg/ml, 25 μg/ml and 50 μg/ml) of affinity-purified anti-GLURP.R0 antibodies (C). Parasite growth was monitored by flow cytometry after CMXRos staining. Horizontal lines represent median SGI values.