Figure 2

PfCHA Ca2+/H + antiporter function in yeast is inhibited by bafilomycin A1. Cytoplasmic Ca²⁺ dependent apoaequorin luminescence was measured following pre-incubation with the V-type ATPase inhibitor bafilomycin A1 (BF). Inhibitor-free controls contained concentrations of solvent (DMSO) equivalent to the solvent present in the BF sample. A. Data from the parental BY4741n strain and the vcx1Δ mutant carrying the plasmid only (vcx1Δ + p505). B. vcx1Δ carrying the yeast VCX1 (vcx1Δ + VCX1) or PfCHA (vcx1Δ + PfCHA). Time of the addition of CaCl₂ is indicated by the arrows. Data represent means of three experiments (n = 3).