Evaluation of dihydrofolate reductase and dihydropteroate synthetase genotypes that confer resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum in Haiti

Table 2 Primer sequences used for nested PCR protocol and sequencing

Gene	Primer	Sequence^‡	Nucleotide location
dhfr	PCR1	M1*	TTTATGATGGAACAAGTCTGC	−3 to 18
		M5*	AGTATATACATCGCTAACAGA	625 to 645
	PCR2	M3*	TTTATGATGGAACAAGTCTGCGACGTT	−3 to 24
	PCR2	F/*	AAATTCTTGATAAACAACGGAACCTTTTA	491 to 519
dhps	PCR1	R2*	AACCTAAACGTGCTGTTCAA	223 to 242
		R/*	AATTGTGTGATTTGTCCACAA	913 to 933
	PCR2	K*	TGCTAGTGTTATAGATATAGGATGAGCATC	269 to 298
	PCR2	K/*	CTATAACGAGGTATTGCATTTAATGCAAGAA	676 to 706
	Seq	Kb^†	ATTGGTTTCGCATCACATTT	408 to 427

‡Primer sequences listed as forward primer first followed by reverse primer. All primers listed 5’ to 3’.
*Primers taken from Duraisingh et al.[27].
†Primer designed for nested sequencing of dhps codon 436 and 437.

ISSN: 1475-2875