Development, validation and evaluation of a rapid PCR-nucleic acid lateral flow immuno-assay for the detection of Plasmodium and the differentiation between Plasmodium falciparum and Plasmodium vivax

Table 3 Results of the ring trial per sample type and laboratory

Sample type	Laboratory 1	Laboratory 2	Laboratory 3	Kappa
P. falciparum	12/13*	12/13*	10/13*	0.873
P. vivax	7/9	8/9	7/9	0.727
P. ovale	4/4	4/4	3/4	0.836^{^}
P. malariae	4/4	4/4	4/4
P. falciparum/P. vivax	1/1	1/1	0/1^$	0.497
Negative^#	61/63	62/63	62/63	0.858

^> Including two P. falciparum culture samples.
*One sample failed in all three laboratories.
^# The two water controls the laboratories added themselves are not presented in the table.
^$ This sample was identified as a P. falciparum mono-infection.
^{^} The results for P. ovale and P. malariae samples were scored as positive, non-falciparum, non-vivax and are therefore combined in the analysis.